Chapter 1
Basics of Physiological-Pharmacological Experimentation
Unit 1 Objectives and Requirements of Physiological-Pharmacological Experiment
Unit 2 Process of Experiment Data and Production of Experiment Reports
Unit 3 Requirements and Safety Rules in Laboratory
Unit 4 Operation of Biological Data Acquisition System RM-6240
Chapter 2
General Knowledge of Physiological-Pharmacological Experimentation
Unit 1 Fundamental Principle of Physiological-Pharmacological Experi- mentation
Unit 2 Preliminary Tests in Physiological-Pharmacological Experimentation
Unit 3 Statistical Analysis Most Often Used in Scientific Activities
Unit 4 Statistic Analysis Using Excel
Chapter 3
Basic Operations on Experimental Animals
Unit 1 Fundamentals of Animal Experiments
Unit 2 Operations on ExperimentalAnimals
Unit 3 Gender Identification and Labeling the Animals
Unit 4 Narcotics and Animal Narcosis
Unit 5 Sample Collection and Bleeding
Unit 6 Drug Administration for Laboratory Animals
Chapter 4
Physiological-Pharmacological Experiments
Experiment 1 Generation and Conduction of Compound Action Potential
Experiment 2 Actions of Antiepileptic and Anticonvulsants in Mouse Models
Experiment 2-1 Effect of Drugs on Electric Convulsive Shock
Experiment 2-2 Effect of Drugs on Convulsion Induced by Central Stimulants
Experiment 3 Actions of Analgesics in Mouse Models
Experiment 3-l Analgesic Effect Assayed by Hot-Plate Model
Experiment 3-2 Analgesic Effect Assayed Using Chemical-Stimulation Model
Experiment 4 Influence of Stimulus on the Contraction of Skeletal Muscle
Experiment 5 Determination of ABO Blood Group
Experiment 6 Effects of Physical and Chemical Factors on Blood Coagulation
Experiment 7 Recording of Electrocardiogram in Toads
Experiment 8 Effects of Chemical Agents on the Activity of the Isolated Toad Heart
Experiment 9 Regulation of Artery Blood Pressure
Expenment 10 Measurement of Human Blood Pressure
Experiment 11 Toxic Effects of Cardiac Glycosides on Heart
Experiment 12 Effect of Drugs on Isolated Intestinal Smooth Muscles
Experiment 13 Regulation of Respiratory Movements
Experiment 14 Experimental Pulmonary Edema in Rabbits
Experiment 15 Factors and Drugs Influencing Urine Output
Experiment 16 Drug Actions Influenced by Dosage Forms and Delivery Routes
Experiment 17 Determination of 50% Effective Dose of Pentobarbital
Experiment 18 Measurement of Concentration-Time Profile of Metronidazole
Experiment 19 Calculation of the Pharmacokinetic Parameters
Experiment 20 Intoxication and Detoxification of Organophosphate Agents
Experiment 21 Effect of Glucocorticoids on Intraabdominal Capillary Permeability in Mice
Experiment 22 Effect of Anti-inflammatory Agents on Tumescence in Rat Model
Experiment 23 In-Vitro Experiment of AntibacterialActions
Chapter 5
Expanded Experiments
Experiment 1 Extraction of Genomic DNA in Peripheral Blood
Experiment 2 Extraction of mRNA from Liver Tissues
Experiment 3 Assessment of Gene Transcription Level by Real-Time PCR
Experiment 4 Assessment of Protein Expression by Western-Blot
Experiment 5 Isolation and Culture of Mouse Hepatocytes
Appendix
Table 1 Physiological parameters of experimental animals
Table 2 Age, body weight and lifetime of adult experimental animals
Table 3 Formularies of commonly used saline solution
Table 4 Bleeding methods for experimental animals
Table 5 Safe and lethal bleeding volume of experimental animals
Table 6 Scaling factors for cross-species dose calculation
Table 7 Application route and dose of commonly used anaesthetics
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